How to prepare stock solution from powder

how to prepare stock solution from powder

HEPES Stock Solution (0.1 M, pH 7.4)

I need to prepare mL of a 10 mM drug stock solution. The drug is a small organic compound with a molecular weight of about g/mol. The problem is that I must dissolve about mg of the. M refers to moles of HCl per 1 liter of water, so if you have 1 liter of water, you simply have to add the corresponding amount of HCl (s). In this case, moles of HCl would be the equivalent of g of said solid. To actually create.

The purpose of this protocol is to prepare a 0. HEPES is a buffer that can be used to control the pH t many solutions, and this particular buffer is used in our lab to make assay buffers for fluorogenic substrate assays to measure enzyme activity in the presence of various inhibitory substances. Farhat, Y. Dec Your email address will not be published. Support the Protocol Place by leaving comments, feedback or suggestions.

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Your Message. Protocol Place. Skip to content. Baker, Deionized water Normal lab equipment, such as a graduated cylinder, small chemical spatula, beakers, stir plate, stir rod, a pH meter, a scale Procedure Add 2. Add about 80 mL of deionized water to the beaker. Begin monitoring pH of the solution. Add one NaOH pellet to raise the pH towards 7. It should reach about pH of 7 Once the first pellet is fully dissolved, add a second NaOH pellet if necessary to raise the pH to 7.

Monitor carefully, and if the pH approaches 7. Note: In my experience, about 1. Sterile-filter, if possible, and store in the refrigerator for up to 4 months or aliquot and freeze at C for future use.

Citing this Protocol Farhat, Y. Leave a Reply Cancel splution Your email address will not be published. Search This Website. Search for:. We especially want to know whether -You used a particular protocol -You found the information or presentation of information to be helpful -The protocol worked as you expected -Something was confusing or unclear Your input will help make this resource better for everyone. Thanks, Youssef Farhat. Theme: Coraline by Automattic.

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The purpose of this protocol is to prepare a M HEPES stock solution at pH HEPES is a buffer that can be used to control the pH of many solutions, and this particular buffer is used in our lab to make assay buffers for fluorogenic substrate assays to measure enzyme activity in the presence of various inhibitory substances. Silicon is a chemical element with the symbol Si and atomic number It is a hard, brittle crystalline solid with a blue-grey metallic lustre, and is a tetravalent metalloid and ledidatingstory.com is a member of group 14 in the periodic table: carbon is above it; and germanium, tin, and lead are below it. It is relatively unreactive. Because of its high chemical affinity for oxygen, it was not. Mar 20,  · Figure \(\PageIndex{3}\): Preparation of a Solution of Known Concentration by Diluting a Stock Solution. (a) A volume (V s) containing the desired moles of solute (M s) is measured from a stock solution of known concentration. (b) The measured volume of stock solution is transferred to a second volumetric flask.

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